NCCN Guidelines for Patients® | Mantle Cell Lymphoma

13 NCCN Guidelines for Patients ® : Mantle Cell Lymphoma, 2017 1 Mantle cell lymphoma basics Diagnosis Flow cytometry Flow cytometry is a newer method that can also be used to assess the surface proteins on lymphoma cells. This method involves first adding a marker—a light-sensitive dye—to cells. Then, your blood will be passed through a flow cytometry machine. The machine measures surface proteins on thousands of cells. Flow cytometry may be done in addition to an IHC panel. If done, it should test for CD5, CD10, CD19, CD20, CD23, and kappa and lambda light chain proteins. Light chain proteins are part of antibodies. It may be helpful to test for CD200 if the type of cancer is unclear. Mantle cell lymphoma cells do not have CD200. Chronic lymphocytic leukemia cells do. Genetic tests Mantle cell lymphoma has common abnormal changes in chromosomes and genes. A translocation is a switching of parts between two chromosomes. A gene rearrangement is the fusion of one gene with another gene to create a new gene. Genetic testing may be useful. t(11;14) Besides a protein signature, mantle cell lymphomas often have a translocation. The translocation in mantle cell lymphoma occurs between chromosomes 11 and 14—referred to as t(11;14). See Figure 5 . t(11;14) often involves the switching of the CCND1 gene on chromosome 11 with the IgH locus on chromosome 14. The result is too much cyclin D1. If cyclin D1 is normal on the IHC panel, it may be useful to test for t(11;14). Figure 5 t(11;14) A translocation is a switching of parts between chromosomes. In mantle cell lymphoma cells, a translocation between chromosomes 11 and 14 is often present. The result is too much cyclin D1 on the surface of cancer cells. Copyright © 2017 National Comprehensive Cancer Network ® (NCCN ® ). www.nccn.org

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